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We recommend to have at least an 8-core Intel/AMD x86_64 processor.

Memory requirements are highly dependent on the input data and the degree of parallelism, but we recommend to have at least 32 GB of memory available.

The disk capacity available at runtime should be several times the size of the FASTQ library files to be analyzed.


ARM processors (e.g. Apple M series, Amazon Graviton, Ampere) are currently not supported.

Operating system

nf-core/pixelator can be run on any POSIX compatible system (Linux systems, macOS). On Windows machines the pipeline can be run using the WSL2 integration layer.


Nextflow requires Bash 3.2 (or later) and Java 11 (or later, up to 18) to be installed.