Technote comparison of MPX, SCSP v1.0 vs v2.0 Kit, Immunology Panel I and II
This dataset is the basis of the technical comparison of MPX V1 vs V2. The Human Immunology panel I and panel II, samplesheets, and MPX library FASTQ files are provided below, as well as the Pixelator output files.
PBMC extraction from whole-blood
Human peripheral blood mononuclear cells (PBMCs) were isolated from a buffy coat by Lymphoprep™️ density gradient centrifugation followed by red blood cell lysis using eBioscience™️ 1X RBC Lysis Buffer for 15 minutes. The platelet fraction was reduced by 2 repeated centrifugation steps at 100 x g for 10 minutes.
The buffy coat, drawn from a healthy volunteer with informed consent and withheld sample identity, was purchased from a Karolinska Hospital blood bank.